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aa 217 548 cc1  (Addgene inc)


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    Structured Review

    Addgene inc aa 217 548 cc1
    Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
    Aa 217 548 Cc1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 2 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aa 217 548 cc1/product/Addgene inc
    Average 93 stars, based on 2 article reviews
    aa 217 548 cc1 - by Bioz Stars, 2026-06
    93/100 stars

    Images

    1) Product Images from "An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts"

    Article Title: An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts

    Journal: Journal of Cell Science

    doi: 10.1242/jcs.263766

    Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor GST–p150-CC1. n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
    Figure Legend Snippet: Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor GST–p150-CC1. n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.

    Techniques Used: Activity Assay, Control, Imaging

    Noncanonical asters can merge. (A) Confocal time-lapse montage of microtubule and EB1–GFP dynamics in egg extracts, showing that the centers of two noncanonical asters merged with each another spontaneously, and that the EB1–GFP-enriched regions at the centers also merged. Each image is a maximum-intensity projection of four confocal planes spanning 6 µm of depth. Imaging started at an arbitrary time point after the asters had formed but had not merged. The plot below each image is the fluorescence intensity profile along a 1.65 µm thick, 22.8 µm long line segment (yellow dashed rectangle) that starts at the bottom left and ends at the top right. For each point on the curve in the plot, the horizontal coordinate is the distance from the start of the line segment, and the vertical coordinate is the average fluorescence intensity of the pixels across the width of the line segment at that distance (a.u., arbitrary units). The black arrows indicate intensity peaks for microtubule (second row) and EB1–GFP (fourth row) fluorescence at the aster centers. n =9. (B) Confocal images of microtubules in control and GST–p150-CC1-treated extracts, showing that noncanonical asters still merged when dynein was inhibited by 2 µM GST–p150-CC1. n =2.
    Figure Legend Snippet: Noncanonical asters can merge. (A) Confocal time-lapse montage of microtubule and EB1–GFP dynamics in egg extracts, showing that the centers of two noncanonical asters merged with each another spontaneously, and that the EB1–GFP-enriched regions at the centers also merged. Each image is a maximum-intensity projection of four confocal planes spanning 6 µm of depth. Imaging started at an arbitrary time point after the asters had formed but had not merged. The plot below each image is the fluorescence intensity profile along a 1.65 µm thick, 22.8 µm long line segment (yellow dashed rectangle) that starts at the bottom left and ends at the top right. For each point on the curve in the plot, the horizontal coordinate is the distance from the start of the line segment, and the vertical coordinate is the average fluorescence intensity of the pixels across the width of the line segment at that distance (a.u., arbitrary units). The black arrows indicate intensity peaks for microtubule (second row) and EB1–GFP (fourth row) fluorescence at the aster centers. n =9. (B) Confocal images of microtubules in control and GST–p150-CC1-treated extracts, showing that noncanonical asters still merged when dynein was inhibited by 2 µM GST–p150-CC1. n =2.

    Techniques Used: Imaging, Fluorescence, Control



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    aa 217 548 cc1  (Addgene inc)
    93
    Addgene inc aa 217 548 cc1
    Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor <t>GST–p150-CC1.</t> n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.
    Aa 217 548 Cc1, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/aa 217 548 cc1/product/Addgene inc
    Average 93 stars, based on 1 article reviews
    aa 217 548 cc1 - by Bioz Stars, 2026-06
    93/100 stars
    		  Buy from Supplier

    Image Search Results


    Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor GST–p150-CC1. n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.

    Journal: Journal of Cell Science

    Article Title: An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts

    doi: 10.1242/jcs.263766

    Figure Lengend Snippet: Formation of the noncanonical aster requires the microtubule motor MKLP2 and Aurora kinase B activity. (A) Confocal images of microtubule organization in control extracts and extracts with 100 µM MKLP2 inhibitor paprotrain. n =4. Each image is a maximum-intensity projection of nine confocal planes spanning 24 μm of depth. (B) Confocal images of microtubule dynamics in control extracts (top row) and extracts with 40 µM Aurora kinase B inhibitor barasertib (bottom row). n =6. Each image is a maximum-intensity projection of nine confocal planes spanning 16 µm of depth. For both A and B, imaging started at an arbitrary time point when asters had just begun to form in the untreated extracts. (C) Widefield epifluorescence images of microtubule organization in control extracts and extracts with 100 µM kinesin Eg5 inhibitor STLC. n =10. (D) Confocal images of microtubule organization in control extracts and extracts with 2 µM dynein inhibitor GST–p150-CC1. n =6. (E) Widefield epifluorescence images of microtubule and ER organization in control extracts and extracts with 0.68 µM GST–p150-CC1. n =2.

    Article Snippet: The chicken DCTN1 p150Glued AA 217–548 (CC1) was from the plasmid pVEX-CC1 (Addgene plasmid 74170; http://n2t.net/addgene:74170 ; RRID:Addgene_74170; deposited by Trina Schroer).

    Techniques: Activity Assay, Control, Imaging

    Noncanonical asters can merge. (A) Confocal time-lapse montage of microtubule and EB1–GFP dynamics in egg extracts, showing that the centers of two noncanonical asters merged with each another spontaneously, and that the EB1–GFP-enriched regions at the centers also merged. Each image is a maximum-intensity projection of four confocal planes spanning 6 µm of depth. Imaging started at an arbitrary time point after the asters had formed but had not merged. The plot below each image is the fluorescence intensity profile along a 1.65 µm thick, 22.8 µm long line segment (yellow dashed rectangle) that starts at the bottom left and ends at the top right. For each point on the curve in the plot, the horizontal coordinate is the distance from the start of the line segment, and the vertical coordinate is the average fluorescence intensity of the pixels across the width of the line segment at that distance (a.u., arbitrary units). The black arrows indicate intensity peaks for microtubule (second row) and EB1–GFP (fourth row) fluorescence at the aster centers. n =9. (B) Confocal images of microtubules in control and GST–p150-CC1-treated extracts, showing that noncanonical asters still merged when dynein was inhibited by 2 µM GST–p150-CC1. n =2.

    Journal: Journal of Cell Science

    Article Title: An acentrosomal aster with atypical microtubule polarity recruits cytokinesis signals to its center in Xenopus egg extracts

    doi: 10.1242/jcs.263766

    Figure Lengend Snippet: Noncanonical asters can merge. (A) Confocal time-lapse montage of microtubule and EB1–GFP dynamics in egg extracts, showing that the centers of two noncanonical asters merged with each another spontaneously, and that the EB1–GFP-enriched regions at the centers also merged. Each image is a maximum-intensity projection of four confocal planes spanning 6 µm of depth. Imaging started at an arbitrary time point after the asters had formed but had not merged. The plot below each image is the fluorescence intensity profile along a 1.65 µm thick, 22.8 µm long line segment (yellow dashed rectangle) that starts at the bottom left and ends at the top right. For each point on the curve in the plot, the horizontal coordinate is the distance from the start of the line segment, and the vertical coordinate is the average fluorescence intensity of the pixels across the width of the line segment at that distance (a.u., arbitrary units). The black arrows indicate intensity peaks for microtubule (second row) and EB1–GFP (fourth row) fluorescence at the aster centers. n =9. (B) Confocal images of microtubules in control and GST–p150-CC1-treated extracts, showing that noncanonical asters still merged when dynein was inhibited by 2 µM GST–p150-CC1. n =2.

    Article Snippet: The chicken DCTN1 p150Glued AA 217–548 (CC1) was from the plasmid pVEX-CC1 (Addgene plasmid 74170; http://n2t.net/addgene:74170 ; RRID:Addgene_74170; deposited by Trina Schroer).

    Techniques: Imaging, Fluorescence, Control